Journal: Frontiers in Molecular Biosciences

Article Title: Expression and distribution of activin-follistatin-inhibin axis in the urinary bladder

doi: 10.3389/fmolb.2025.1519977

Figure Lengend Snippet: Primers used for RT-qPCR.

Article Snippet: Acvr1 , Proteintech , 67417-1-Ig , IF, 1:100.

Techniques: Sequencing

Journal: Frontiers in Molecular Biosciences

Article Title: Expression and distribution of activin-follistatin-inhibin axis in the urinary bladder

doi: 10.3389/fmolb.2025.1519977

Figure Lengend Snippet: Antibody list.

Article Snippet: Acvr1 , Proteintech , 67417-1-Ig , IF, 1:100.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Expression and distribution of activin-follistatin-inhibin axis in the urinary bladder

doi: 10.3389/fmolb.2025.1519977

Figure Lengend Snippet: mRNA expression of AFI axis family members in the bladder of male and female mice. (A) Differences in mRNA expression of Inha, Inhba, Inhbb, Inhbc, Inhbe, and Fst in the bladder of male and female mice. (B) Differences in mRNA expression of Acvr1, Acvr1b, Acvr1c, Acvr2a, and Acvr2b in the bladder of male and female mice (n = 3, Data were analyzed with the use of Student’s t-test, **p < 0.01, ***p < 0.001).

Article Snippet: Acvr1 , Proteintech , 67417-1-Ig , IF, 1:100.

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Expression and distribution of activin-follistatin-inhibin axis in the urinary bladder

doi: 10.3389/fmolb.2025.1519977

Figure Lengend Snippet: Protein expression of AFI axis family members in the bladder of male and female mice. (A–G) Western blot analysis of INHA (A) , INHBA (B) , INHBE (C) , FST (D) , ACVR1 (E) , ACVR1B (F) , ACVR2B (G) in both male and female mouse bladder (25 μg protein loaded). (H) Differences in protein expression of AFI axis family members in the bladder of male and female mice. (n = 4, molecular values of major detected protein species are indicated at the right of each panel. Data were analyzed with the use of a two-tailed Student’s t-test, **p < 0.01, ***p < 0.001).

Article Snippet: Acvr1 , Proteintech , 67417-1-Ig , IF, 1:100.

Techniques: Expressing, Western Blot, Two Tailed Test

Journal: Frontiers in Molecular Biosciences

Article Title: Expression and distribution of activin-follistatin-inhibin axis in the urinary bladder

doi: 10.3389/fmolb.2025.1519977

Figure Lengend Snippet: Localization of ACVR1 in mouse bladder. Cryosections of mouse bladder tissue were labeled with ACVR1 antibody (green) and ALPL (A) , or antibodies to ENTPD3 (B) or KRT5 (C) ; all displayed in red. Nuclei were labeled with DAPI (blue). Far right : merged images. L, bladder lumen. U, urothelium. Scale bars = 10 μm.

Article Snippet: Acvr1 , Proteintech , 67417-1-Ig , IF, 1:100.

Techniques: Labeling

Journal: International journal of molecular sciences

Article Title: Long-Term Changes in Ovarian Follicles of Gilts Exposed Neonatally to Methoxychlor: Effects on Oocyte-Derived Factors, Anti-Müllerian Hormone, Follicle-Stimulating Hormone, and Cognate Receptors.

doi: 10.3390/ijms23052780

Figure Lengend Snippet: Figure 2. ACVR1 (a,a’), BMPR1A (b,b’), BMPR1B (c,c’), and TGFBR1 (d,d’) mRNA and protein abundance in preantral (primordial, primary, and secondary follicles) and small antral follicles obtained from the control (CTR) and methoxychlor (MXC-) treated gilts. The mRNA expression (quantitative real-time PCR) is presented relative to GAPDH as mean ± SEM (a–d). Relative protein abundance was measured by the densitometric method and expressed as the ratio relative to β-actin abundance (mean ± SEM, a’–d’). The fragment of membranes with bands corresponding to predicted molecular weights is shown above graphs. Asterisks denote significant differences between the CTR and treated animals (for preantral follicles pool n = 5, for small antral follicle n = 15, * p < 0.05, ** p < 0.01, *** p < 0.001, Mann–Whitney U test).

Article Snippet: Next, the membranes were incubated for 1 h at room temperature with secondary anti-rabbit (in the case of AMH, AMHR2, BMP15, BMPR1A, BMPR1B, BMPR2, FSHR, GDF9, and TGFBR1) or anti-goat (for ACVR1) antibodies linked to horseradish peroxidase (Jackson ImmunoResearch, Cambridge, UK) at 1:10000 antibody dilution.

Techniques: Quantitative Proteomics, Control, Expressing, Real-time Polymerase Chain Reaction, MANN-WHITNEY

Journal: International journal of molecular sciences

Article Title: Long-Term Changes in Ovarian Follicles of Gilts Exposed Neonatally to Methoxychlor: Effects on Oocyte-Derived Factors, Anti-Müllerian Hormone, Follicle-Stimulating Hormone, and Cognate Receptors.

doi: 10.3390/ijms23052780

Figure Lengend Snippet: Figure 3. Immunolocalization of ACVR1, BMPR1A, BMPR1B, and TGFBR1 in preantral (primordial, primary, and secondary follicle) (a) and small antral (b) follicles of the control (CTR) and methoxychlor (MXC-) treated gilts. ACVR1, BMPR1A, BMPR1B, and TGFBR1 positive staining was observed in oocytes (asterisks) and granulosa cells (arrows) of preantral follicles, as well as granulosa (arrows) and theca cells (arrowheads) of small antral follicles, in both examined groups. All sections were counterstained with hematoxylin QS. There was no positive staining observed in the negative control sections (a,b, insets). Prim—primordial follicles; SF—secondary follicles; AF—antral follicles. Bars = 50 µm.

Article Snippet: Next, the membranes were incubated for 1 h at room temperature with secondary anti-rabbit (in the case of AMH, AMHR2, BMP15, BMPR1A, BMPR1B, BMPR2, FSHR, GDF9, and TGFBR1) or anti-goat (for ACVR1) antibodies linked to horseradish peroxidase (Jackson ImmunoResearch, Cambridge, UK) at 1:10000 antibody dilution.

Techniques: Control, Staining, Negative Control

Journal: Biology

Article Title: BMP-2-Driven Osteogenesis: A Comparative Analysis of Porcine BMSCs and ASCs and the Role of TGF-β and FGF Signaling.

doi: 10.3390/biology14060610

Figure Lengend Snippet: Figure 6. Receptor expressions of ALK2, ALK4, ALK6, ALK5, ALK7, BMPR-II in pASC. Grouped representation of the respective receptor expression in the course of osteogenic differentiation (OM +/−BMP-2). From day 19, there is a significant induction of ALK 2, ALK 6, and ALK 5 with the addition of BMP-2. BMPR-II expression in the OM group decreased in OM and tended to stay increased under BMP-2 supplementation from day 19, but was not considered significant (* p ≤0.05, ** p ≤0.01; n = 6, BMP-2 450 ng/mL).

Article Snippet: The respective conjugated antibodies were used for the expressions of ALK3 (Cat. No.: AF436), ALK 5 (Cat. No.: FAB5871), ALK6 (Cat. No.: FAB5051A), TGF-β2-RII (Cat. No.: FAB532P), ALK7 (Cat. No.: FAB77491A), ALK2 (Cat. No.: AF637), ALK4 (Cat. No.: MAB2221), and BMPR-II (Cat. No.: AF811) (by R&D Systems, Minneapolis, MN, USA), and the pASCs and pBMSCs were compared for their expressions of the specific surface antigens CD45 (Cat. No.: MCA1568GA, BioRad, Hercules, CA, USA), HLA-DR (human leukocyte antigen–antigen D-related surface molecule) (Cat. No.: MCA2314F, Bio-Rad, Hercules, CA, USA), CD29 (Cat. No.: 561,496, BD Pharmingen, Franklin Lakes, NJ, USA), CD79alpha (Bio-Rad, Cat. No.: MCA2538GA), CD14 (Cat. No.: MCA1568GA, Bio-Rad, Hercules, CA, USA), CD31 (Cat. No.: AF3387, R&D Systems, Minneapolis, MN, USA), CD105 (Cat. No.: NB110-58718APC, Novus Biologicals, Minneapolis, MN, USA), CD26 (, Cat. No.: NB600-552APC, Novus Biologicals, Minneapolis, MN, USA), CD73 (, Cat. No.: AF4488, R&D Systems, Minneapolis, MN, USA), CD90 (Cat. No.: 559,869, BD Pharmingen, Franklin Lakes, NJ, USA), CD34 (Cat. No.: 81289, abcam, Cambridge, UK), and CD44 (Cat. No.: 5531, BD Pharmingen, Franklin Lakes, NJ, USA).

Techniques: Expressing